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This capability is desperately needed to match the explosive growth in high-parameter single-cell analysis in the Cambridge biomedical community (high-parameter flow-cytometry, CyTOF mass-spec, single-cell (sc)-RNA-seq). Parallel cell-sorting capacity of mouse and human live-cells is crucial for validation of findings, and in-depth exploration of the biology of highly purified cell populations. Currently, there is no capacity in Cambridge for high-parameter CL2-designated cell-sorting. This Wellcome Trust multi-user equipment grant application will provide a critical resource to the Cancer Research UK, Cambridge Institute (CRUK-CI), and wider Cambridge biomedical community.","language":"en"},{"description":"Our bodies are composed of trillions of individual cells, all of which work in concert to achieve our form and function. Using new technologies, we can now more reliably classify individual cells, which has revealed a profound diversity within previously-believed uniform populations. Studying how these diverse cell-types interact within their environment is central to our understanding of how our bodies function, or malfunction in disease. To investigate the roles of these diverse cell-types, we propose to use a technology called fluorescence activated cell-sorting (FACS), which is highly adaptable for multiple uses. We will use our expertise in FACS, as well as other leading technologies, to purify live cells from complex, rare human (and other) samples to ask questions about their individual roles in health and disease. Thus, the proposed FACS machine will help both clinicians and basic scientists in Cambridge focus important research on very specific cell-types in our bodies.","language":"en"}],"investigator":[{"given":"Carlos","family":"Caldas","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]},{"given":"Duncan","family":"Jodrell","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7764-577X","authenticated-orcid":false,"given":"Masashi","family":"Narita","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054225q67","id-type":"ROR","asserted-by":"publisher"}],"name":"Cancer Research UK","country":"GB"}]},{"given":"Rahul","family":"Roychoudhuri","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01d5qpn59","id-type":"ROR","asserted-by":"publisher"}],"name":"Babraham Institute","country":"GB"}]},{"given":"Elisa","family":"Laurenti","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4021-3898","authenticated-orcid":false,"given":"Greg","family":"Hannon","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0558-4119","authenticated-orcid":false,"given":"Maike","family":"de la Roche","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054225q67","id-type":"ROR","asserted-by":"publisher"}],"name":"Cancer Research UK","country":"GB"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5200-2698","authenticated-orcid":false,"given":"Naomi","family":"McGovern","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]}],"lead-investigator":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9773-0023","authenticated-orcid":false,"given":"Timotheus","family":"Halim","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Cambridge","country":"GB"}]}],"award-amount":{"amount":479450.0,"currency":"GBP"},"award-start":{"date-parts":[[2019,11,1]]},"award-end":{"date-parts":[[2024,10,31]]},"funding":[{"type":"equipment","scheme":"Cell and Developmental Biology","award-amount":{"amount":479450.0,"currency":"GBP","percentage":100},"funder":{"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}],"name":"Wellcome Trust"}}]}],"deposited":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T16:27:08Z","timestamp":1739896028000},"score":1,"resource":{"primary":{"URL":"https:\/\/europepmc.org\/grantfinder\/grantdetails?query=pi:\"%7BProfessor%7D%7BCaldas%7D%7BCarlos%7D%7BC%7D\" gid:\"218300\" ga:\"Wellcome Trust\""}},"issued":{"date-parts":[[2019,11,1]]},"URL":"https:\/\/doi.org\/10.35802\/218300","relation":{},"subject":[]}}