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WGS has been routinely available in the National Health Service (NHS) England for all children with cancer in England since 2021, but its uptake has been variable geographically. To explore the underlying barriers to routine use of WGS in this population across England and more widely in the United Kingdom (UK) and the Republic of Ireland (ROI), a one-day workshop was held in Cambridge, United Kingdom in October 2022.<\/jats:p>\n <\/jats:sec>\n Methods<\/jats:title>\n Following a series of talks, delegates participated in open, round-table discussions to outline local and broader challenges limiting routine WGS for diagnostic work-up for children with cancer in their Principal Treatment Centres (PTCs) and Genomic Laboratory Hubs (GLHs). Within smaller groups, delegates answered structured questions regarding clinical capability, education and training needs, and workforce competence and requirements. Data was recorded, centrally collated, and analysed following the event using thematic analysis.<\/jats:p>\n <\/jats:sec>\n Results<\/jats:title>\n Sixty participants attended the workshop with broad representation from the 20 PTCs across the UK and ROI and the seven GLHs in England. All healthcare professionals involved in the WGS pathway were represented, including paediatric oncologists, clinical geneticists, clinical scientists, and histopathologists. The main themes highlighted by the group in ensuring equitable access to WGS identified were: lack of knowledge equity between NHS trusts, with a perception of WGS being for research only; and perception of lack of financial support for the clinical process surrounding WGS, including lack of time to take informed consent from patients. The latter also included limited trained staff available for data interpretation, affecting the turnaround time for reporting. Finally, the need for an integrated digital pathway to order, track, and return data to clinicians was highlighted.<\/jats:p>\n <\/jats:sec>\n Conclusion<\/jats:title>\n At the workshop, the general motivation for including WGS in the diagnostic work up for children with cancer was high throughout the UK, however a perceived lack of resources and education opportunities limit the widespread use of this commissioned assay. This workshop has led to some recommendations to increase access to WGS in this population in England and more widely in the devolved national of the UK and the ROI.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12909-024-06219-y","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T19:01:46Z","timestamp":1730919706000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identifying barriers and opportunities to facilitate the uptake of whole genome sequencing in paediatric haematology and oncology practice"],"prefix":"10.1186","volume":"24","author":[{"given":"Michelle","family":"Bishop","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Vedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Bowdin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Bartram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bentley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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This Research Governance team have confirmed that this study does not constitute research and as such no further review was required from the organisation. Participants were informed in the original email invitation and in pre-workshop papers that de-identified data would be collected during the workshop and that this data would be analysed and published. This information stated that agreeing to attend and attendance on the day would be taken as consent for the data to be collected, analysed and reported. This information was also repeated in person at the beginning of the workshop. MB was present at the workshop to answer any questions and address any concerns including removal of any data from participants who preferred their de-identified information not be included in any reports.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1273"},{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:25:28Z","timestamp":1780705528069,"version":"3.54.1"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["221616"],"award-info":[{"award-number":["221616"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Antimicrob Resist Infect Control"],"abstract":"Abstract<\/jats:title>\n The use of antimicrobials in Thailand has been reported as one of the highest in the world in human and animal sectors. Our engagement project aimed to improve our understanding of the issue of antimicrobial use and antimicrobial resistance (AMR) among adult Thai communities, and co-create locally relevant solutions to AMR, especially those focusing on raising awareness to improve related policies in Thailand.<\/jats:p>\n We conducted a series of online and in-person \u2018conversations\u2019 according to Wellcome\u2019s \u2018Responsive Dialogues\u2019 engagement approach, designed to bring together different voices to understand complex AMR problems and find potential solutions. This approach enabled key AMR stakeholders and policy makers to hear directly from communities and members of the public, and vice versa. Conversations events took place between 25 November 2020 and 8 July 2022, and we engaged 179 AMR key stakeholders and members of the public across Thailand.<\/jats:p>\n The issues found were: there were quite a lot of misunderstandings around antimicrobials and AMR; participants felt that communications and engagement around antimicrobial resistance had limited reach and impact; asking for and taking antibiotics for self-limiting ailments is a social norm in Thailand; and there appeared to be a wide availability of cheap antimicrobials. To mitigate the spread of AMR, participants suggested that the messages around AMR should be tailored to the target audience, there should be more initiatives to increase general health literacy, there should be increased availability of AMR related information at the local level and there should be increased local leadership of AMR mitigation efforts.<\/jats:p>\n \n Trial registration\u00a0<\/jats:bold>\n Thaiclinicaltrials.org registration: TCTR20210528003 (28\/05\/2021).\n <\/jats:p>","DOI":"10.1186\/s13756-024-01416-2","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T08:01:37Z","timestamp":1720080097000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Embedding community and public voices in co-created solutions to mitigate antimicrobial resistance (AMR) in Thailand using the \u2018Responsive Dialogues\u2019 public engagement framework"],"prefix":"10.1186","volume":"13","author":[{"given":"Tassawan","family":"Poomchaichote","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niyada","family":"Kiatying-Angsulee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanpong","family":"Boonthaworn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhensri","family":"Naemiratch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supanat","family":"Ruangkajorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravikanya","family":"Prapharsavat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaiwat","family":"Thirapantu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karnjariya","family":"Sukrung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Direk","family":"Limmathurotsakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Osterrieder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phaik Yeong","family":"Cheah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"issue":"7","key":"1416_CR1","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1179\/2047773215y.0000000030[publishedOnlineFirst:20150907]","volume":"109","author":"F Prestinaci","year":"2015","unstructured":"Prestinaci F, Pezzotti P, Pantosti A. 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However, they face implementation challenges including data accessibility and literacy. Data dashboards offer a method to address these challenges, thereby promoting data-informed practice. This paper reports on a pilot of The School Health Research Network Data Dashboard with secondary schools.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The Dashboard was piloted by three secondary schools (recruited by size; free school meal entitlement) in Wales, UK. Interviews with school staff (\n N<\/jats:italic>\n \u2009=\u20097) and public health practitioners (\n N<\/jats:italic>\n \u2009=\u20096) were conducted. Data were analysed thematically. Research design, including interview questions, analytical interpretation and code, and theme development utilised Complex Adaptive Systems as the conceptual framework.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n School staff had access to multiple data sets for health promotion but often lacked the time and capacity to utilise them. The Dashboard was perceived to be a user-friendly method of enhancing data accessibility and usability in secondary schools, providing appropriate training and guidance for school staff was available to avoid data misinterpretation. National roll-out of the Dashboard was supported if it aligned with the needs of schools and the wider education system.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The Dashboard presents an opportunity for data-informed health and well-being practice and could also support schools to meet education system requirements, providing school staff receive appropriate training. This paper offers novel practical, policy-relevant insights on the interaction of digital dashboards with school systems, capacity constraints, and professional learning needs and valuable insights for public health systems seeking to support data-informed practice in educational settings.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12889-026-26647-3","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T07:25:10Z","timestamp":1771399510000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Piloting a data dashboard to support data-informed health promotion in secondary schools: qualitative analysis of stakeholder interviews"],"prefix":"10.1186","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5288-6614","authenticated-orcid":false,"given":"J.","family":"Van Godwin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1284-9645","authenticated-orcid":false,"given":"S.","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bowen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3388-8902","authenticated-orcid":false,"given":"H.","family":"Reed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4671-2797","authenticated-orcid":false,"given":"N.","family":"Page","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7949-4039","authenticated-orcid":false,"given":"M.","family":"Svobodova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3380-4966","authenticated-orcid":false,"given":"M.","family":"Boffey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9484-1729","authenticated-orcid":false,"given":"F.","family":"Rice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0254-3397","authenticated-orcid":false,"given":"Y.","family":"Shenderovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8976-9825","authenticated-orcid":false,"given":"R.","family":"Bevan-Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3589-3681","authenticated-orcid":false,"given":"S.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6215-0870","authenticated-orcid":false,"given":"J.","family":"Segrott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"issue":"9801","key":"26647_CR1","doi-asserted-by":"publisher","first-page":"1515","DOI":"10.1016\/S0140-6736(11)60827-1","volume":"378","author":"C Kieling","year":"2011","unstructured":"Kieling C, Baker-Henningham H, Belfer M, Conti G, Ertem I, Omigbodun O, et al. 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However, the tissue\u2010level organisation of HES5 dynamics in neurogenesis is unknown. Here, we analyse the expression of HES5\n ex vivo<\/jats:italic>\n in the developing mouse ventral spinal cord and identify microclusters of 4\u20136 cells with positively correlated HES5 level and ultradian dynamics. These microclusters are spatially periodic along the dorsoventral axis and temporally dynamic, alternating between high and low expression with a supra\u2010ultradian persistence time. We show that Notch signalling is required for temporal dynamics but not the spatial periodicity of HES5. Few Neurogenin 2 cells are observed per cluster, irrespective of high or low state, suggesting that the microcluster organisation of HES5 enables the stable selection of differentiating cells. Computational modelling predicts that different cell coupling strengths underlie the HES5 spatial patterns and rate of differentiation, which is consistent with comparison between the motoneuron and interneuron progenitor domains. 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has been a major driving force in the evolution of the human genome. In sub-Saharan African populations, two neighbouring polymorphisms in the Complement Receptor One (CR1) gene, named Sl2 and McCb, occur at high frequencies, consistent with selection by malaria. Previous studies have been inconclusive. Using a large case-control study of severe malaria in Kenyan children and statistical models adjusted for confounders, we estimate the relationship between Sl2 and McCb and malaria phenotypes, and find they have opposing associations. The Sl2 polymorphism is associated with markedly reduced odds of cerebral malaria and death, while the McCb polymorphism is associated with increased odds of cerebral malaria. We also identify an apparent interaction between Sl2 and \u03b1+thalassaemia, with the protective association of Sl2 greatest in children with normal \u03b1-globin. 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We used fMRI and representational similarity analysis (RSA) to identify shared neural representations of spoken language phonological structure. A group of deaf adult participants (N = 22), with a mixture of sign language and spoken language backgrounds and reading abilities, were presented with single lexical items as visual speech and dynamic text (cursive text, revealed letter-by-letter to promote a phonological reading strategy). Adult hearing participants (N = 25) were presented with the same words, but as visual speech and auditory speech. Shared neural representations of phonological structure of English words were found in each group in the superior and middle temporal cortex (STC\/MTC) and these abstract representations were more similar across different language forms in better readers. Our data provide neurobiological evidence of the contribution of visual speech to abstract phonological representations of spoken language, that relate to reading proficiency, in both deaf and hearing adults.<\/jats:p>","DOI":"10.1073\/pnas.2535704123","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:11:09Z","timestamp":1779819069000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Reading ability in both deaf and hearing adults is linked to neural representations of abstract phonology derived from visual speech"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9667-0671","authenticated-orcid":false,"given":"Samuel","family":"Evans","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Cognitive Neuroscience, University College London","place":["London, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Deafness Cognition and Language Research Centre, Division of Psychology and Language Sciences, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy J.","family":"Price","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Imaging Neuroscience, Queen Square Institute of Neurology, University College London","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0264-8532","authenticated-orcid":false,"given":"J\u00f6rn","family":"Diedrichsen","sequence":"additional","affiliation":[{"name":"Western Institute for Neuroscience, Western University","place":["London, Canada"]},{"name":"Department of Computer Science, Western 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S. Beauchamp K. E. Lee B. D. Argall A. Martin Integration of auditory and visual information about objects in superior temporal sulcus. Neuron 41 809\u2013823 (2004).","DOI":"10.1016\/S0896-6273(04)00070-4"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/hbm.26713"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4572-10.2011"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bandl.2009.10.004"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2015.00151"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.08.075"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","unstructured":"I. Simanova P. Hagoort R. Oostenveld M. A. J. van Gerven \u201cModality-independent decoding of semantic information from the human brain\u201d in Cerebral Cortex (New York N.Y.: 1991) (2014) vol. 24 pp. 426\u2013434.","DOI":"10.1093\/cercor\/bhs324"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56972"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1415-24.2025"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1162\/jocn_a_00161"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1057\/9780230625396_6"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/264746a0"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/dev.20032"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2016.02.061"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bandl.2023.105304"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10548-023-00959-8"},{"key":"e_1_3_4_47_2","unstructured":"J. Alegria J. Leybaert C. Hage \u201cThe origin of phonological representations in the deaf\u201d in Language Processing in Spanish M. Carreiras J. E. Garcia-Albea N. Sebastian-Galles Eds. (1992) pp. 107\u2013132."},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1044\/2025_JSLHR-24-00872"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/desc.13124"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/desc.13431"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1044\/2015_JSLHR-S-14-0269"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-7610.00648"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","unstructured":"V. L. Hanson C. A. Fowler Phonological coding in word reading: Evidence from hearing and deaf readers. Mem. Cogn. 15 199\u2013207 (1987).","DOI":"10.3758\/BF03197717"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0327142"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0963-7214.2005.00372.x"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/wcs.1404"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bandl.2013.05.001"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bandl.2016.06.007"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropsychologia.2018.07.010"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1162\/NOL.a.13"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/lnc3.12407"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2006.12.046"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2007.12.047"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tics.2011.04.003"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropsychologia.2007.11.026"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054696"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2007.01.025"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/jdsade\/enag004"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2022.716554"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/deafed\/enw065"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.deafed.a014308"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1353\/sls.2015.0006"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139610"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.3390\/languages8010059"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ridd.2015.10.004"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2113"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2331"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2012.04.062"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2020.03.019"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.00206"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1162\/imag_a_00524"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tics.2011.04.001"},{"key":"e_1_3_4_83_2","first-page":"11","article-title":"Reading comprehension test","volume":"7","author":"Warden V.","year":"1996","unstructured":"V. Warden, Reading comprehension test. Dyslexia Review 7, 11\u201316 (1996).","journal-title":"Dyslexia Review"},{"key":"e_1_3_4_84_2","unstructured":"T. Ellis M. MacSweeney B. Dodd R. Campbell \u201cTAS: A new test of adult speechreading. Deaf people really can be better speechreaders\u201d in International Conference on Auditory-Visual Speech Processing (2001) pp. 1\u20135."},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003553"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2015.12.012"},{"key":"e_1_3_4_87_2","doi-asserted-by":"crossref","unstructured":"S. Evans Reading ability in both deaf and hearing adults is linked to neural representations of abstract phonology derived from visual speech. OSF. https:\/\/osf.io\/cba8z\/overview. Deposited 1 May 2026.","DOI":"10.1101\/2025.11.09.684749"}],"container-title":["Proceedings of the National Academy of Sciences"],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2535704123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:13:09Z","timestamp":1779819189000},"score":9.870893,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2535704123"}},"issued":{"date-parts":[[2026,5,26]]},"references-count":87,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2026,6,2]]}},"alternative-id":["10.1073\/pnas.2535704123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2535704123","ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"published":{"date-parts":[[2026,5,26]]},"assertion":[{"value":"2025-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2535704123"},{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:09:34Z","timestamp":1772168974179,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.73900.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000}}],"reference-count":32,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["214185\/Z\/18\/Z"],"award-info":[{"award-number":["214185\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["103994\/Z\/14\/Z"],"award-info":[{"award-number":["103994\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["074833\/Z\/04\/Z"],"award-info":[{"award-number":["074833\/Z\/04\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wellcome","award":["20517\/Z\/16\/Z"],"award-info":[{"award-number":["20517\/Z\/16\/Z"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"\n Background<\/ns3:bold>\n <\/ns3:p>\n The long-term impact of elevated blood pressure on mortality outcomes has been recently revisited due to proposed changes in cut-offs for hypertension. This study aimed at assessing the association between high blood pressure levels and 10-year mortality using the Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC-7) and the American College of Cardiology and the American Heart Association (ACC\/AHA) 2017 blood pressure guidelines.<\/ns3:p>\n \n Methods<\/ns3:bold>\n <\/ns3:p>\n Data of the PERU MIGRANT Study, a prospective ongoing cohort, was used. The outcome of interest was 10-year all-cause mortality, and exposures were blood pressure categories according to the JNC-7 and ACC\/AHA 2017 guidelines. Log-rank test, Kaplan-Meier and Cox regression models were used to assess the associations of interest controlling for confounders. Hazard ratios (HR) and 95% confidence intervals (95% CI) were estimated.<\/ns3:p>\n \n Results<\/ns3:bold>\n <\/ns3:p>\n A total of 976 records, mean age of 60.4 (SD: 11.4), 513 (52.6%) women, were analyzed. Hypertension prevalence at baseline almost doubled from 16.0% (95% CI 13.7%\u201318.4%) to 31.3% (95% CI 28.4%\u201334.3%), using the JNC-7 and ACC\/AHA 2017 definitions, respectively. Sixty-three (6.4%) participants died during the 10-year follow-up, equating to a mortality rate of 3.6 (95% CI 2.4\u20134.7) per 1000 person-years. Using JNC-7, and compared to those with normal blood pressure, those with pre-hypertension and hypertension had 2-fold and 3.5-fold increased risk of death, respectively. Similar mortality effect sizes were estimated using ACC\/AHA 2017 for stage 1 and stage 2 hypertension.<\/ns3:p>\n \n Conclusions<\/ns3:bold>\n <\/ns3:p>\n Blood pressure levels under two different definitions increased the risk of 10-year all-cause mortality. Hypertension prevalence doubled using ACC\/AHA 2017 compared to JNC-7. The choice of blood pressure cut-offs to classify hypertension categories need to be balanced against the patients benefit and the capacities of the health system to adequately handle a large proportion of new patients.<\/ns3:p>","DOI":"10.12688\/f1000research.73900.2","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T12:40:13Z","timestamp":1673959213000},"page":"1134","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood pressure and 10-year all-cause mortality: Findings from the PERU MIGRANT Study"],"prefix":"10.12688","volume":"10","author":[{"given":"Aida","family":"Hidalgo-Benites","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Senosain-Leon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo M.","family":"Carrillo-Larco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Ruiz-Alejos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam","family":"Smeeth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4738-5468","authenticated-orcid":false,"given":"J. 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Afr. 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We do now understand the age cut-off at year 30 and thank the authors for clarification. Nonetheless, we are wondering how the new categorization is justified. With regards to blood pressure and mortality, we would think that particularly the age group 60+ might be at high risk and thus, more detailed analysis (i.e., splitting into further age group 60-69, 70-79, etc.) might be of interest. We kindly ask the authors for more details on the stratification choice. Overall, though, we still recommend including age as a covariate measured on a continuous scale to improve the power of the analysis.<\/b> \nResponse:<\/i><\/b> The categorization of the age variable is due to the fact that the sampling approach was stratified by age in that way (i.e., 30-39, 40-49, 50-59, and 60+). For instance, even we can adjust for that variable in different forms (i.e., using different cut-offs or in a continuous fashion), the best way would be to adjust for the form the variable was stratified. This was explained in the \u201cSettings and participants\u201d subheading in the Methods section. In addition, this was added in the Statistical analysis subheading: \u201c\nPotential confounders were selected under epidemiological criteria. Since the selection of the participant\u2019s sample was stratified by age (i.e., 30-39, 40-49, 50-59, 60+), such variable was included in that form in adjusted models.<\/i>\u201d \n2. Current guidelines used to report the results of observational studies such as STROBE clearly state that inferential measures should not be used for description of the study population. In line with that, we strongly suggest to delete all p-values from tables 1-3 and to simply describe the characteristics of the study participants. Further, and also as advanced by current guidelines, the use of p-values is not an appropriate criterion for selecting which confounders to adjust for in the analysis. Consequently, we advocate that any statement about potential associations based on single p-values should be avoided.<\/b> \nResponse:<\/i><\/b> We have deleted p-values from tables 1 to 3 as suggested. We have not selected confounders based on p-values, but in an epidemiological approach (pre-specified model). \n3. We thank the authors for rewriting the Statistical analysis section where they refer to the Kaplan-Meier estimator. In the text it now reads that the evaluation of the proportional hazard assumption is based on visual inspection of a graph. For higher transparency and better understanding, we would suggest to include the graph in the article or as supporting material.<\/b> \nResponse:<\/i><\/b>\n <\/b>We have included the figure in the manuscript as requested. We believe the figure does not add more information. Thus, we think the figure should be included in online material but F1000Research does not accept supplemental material. We leave the final decision to include the figure to the reviewer and the editor. \n4. We are pleased to see that the authors followed our recommendation and changed the term \u201cbivariate model\u201d. Though, the term \u201cbivariable model\u201d is still somewhat misleading and rather uncommon to use. We would suggest to simply refer to an \u201cassociation\u201d.<\/b> \nResponse:<\/i><\/b> We have deleted the word bivariable from the text of the manuscript. \n5. As suggested by the authors, we have reviewed the Public health relevance section for more information on how their study provides input e.g. for local decision making or public health implications. From our point of view, the article still lacks precise guidance in which way the estimates\/results from the study presented in the article can be used for decision making with respect to benefits and risks for patients with newly diagnosed hypertension. Consequently, we highly recommend that the authors explain the implications of their study in more detail and that they provide concrete ideas of possible future activities.<\/b> \nResponse:<\/i><\/b> This is an observational study looking at the association between two different guidelines for hypertension definition and all-cause mortality, and not a clinical trial to do decision making. However, according to our results, we have added some lines: \n\u201cTreatment must be guaranteed for hypertension (using JNC-7) and stage 2 hypertension (ACC\/AHA 2017) to reduce mortality risk. If this effect can be seen in pre-hypertension (JNC-7) and stage 1 hypertension (ACC\/AHA 2017) should be further assessed.\u201d<\/i> \n6. In our first review we noted that the authors were not consistent in their writing and with the vocabulary used in the tables, e.g. "stage 1" vs. "stage-1" vs. "stage I" (same applies for \u201cstage 2\u201d). In the reviewed article we still find inconsistencies, particularly comparing the Public health relevance section with the rest of the text.<\/b> \nResponse:<\/i><\/b> We have reviewed through manuscript, tables and figures to keep consistency.","URL":"https:\/\/f1000research.com\/articles\/10-1134\/v2#referee-comment-9541","order":1,"name":"referee-comment-9541","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"RMC-L is supported by a Wellcome Trust International Training Fellowship, London, UK (214185\/Z\/18\/Z). AB-O (103994\/Z\/14\/Z) and JJM (074833\/Z\/04\/Z, 20517\/Z\/16\/Z) were supported by the Wellcome Trust, London, UK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]},{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:29:17Z","timestamp":1781533757374,"version":"3.54.5"},"posted":{"date-parts":[[2026,4,21]]},"group-title":"Cell Biology","reference-count":66,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome","award":["220780"],"award-info":[{"award-number":["220780"]}]},{"name":"Wellcome","award":["319314"],"award-info":[{"award-number":["319314"]}]},{"name":"Wellcome","award":["218470"],"award-info":[{"award-number":["218470"]}]},{"name":"Wellcome","award":["203149"],"award-info":[{"award-number":["203149"]}]},{"name":"Wellcome","award":["226791"],"award-info":[{"award-number":["226791"]}]},{"id":[{"id":"https:\/\/ror.org\/00fzhcx31","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"accepted":{"date-parts":[[2026,4,21]]},"abstract":"Abstract<\/jats:title>\n \n Meiosis generates haploid gametes from a diploid progenitor. In meiosis I, homologous chromosomes segregate while sister chromatids co-orient toward the same spindle pole. The mechanisms underlying this specialized segregation pattern remain incompletely understood. Here we identify pathways required for meiosis I chromosome segregation through a forward genetic screen in\n Saccharomyces cerevisiae<\/jats:italic>\n . We find that meiosis I is highly sensitive to perturbations in diverse components of the segregation machinery and define key functional interfaces within complexes important for this division. These include meiosis I\u2013specific regulators that establish the specialized chromosome pattern, namely the monopolin complex that directs sister kinetochore co-orientation and the Spo13\n MOKIR<\/jats:sup>\n \u2013Cdc5\n Polo<\/jats:sup>\n module that promotes meiosis I chromosome segregation. In addition, we identify mutations affecting the core segregation machinery, including the spindle pole body, spindle midzone, and outer kinetochore, which can disrupt coupling between the chromosome segregation program and the meiotic spindle cycle. Together, our findings reveal that multiple pathways coordinate chromosome segregation with the meiotic divisions and highlight the unique demands of meiosis I.\n <\/jats:p>","DOI":"10.64898\/2026.04.17.719180","type":"posted-content","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:05:16Z","timestamp":1776816316000},"source":"Crossref","is-referenced-by-count":0,"title":["Multiple pathways couple kinetochore orientation to the meiotic spindle cycle"],"prefix":"10.64898","author":[{"given":"Aparna","family":"Vinod","sequence":"first","affiliation":[{"name":"Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, Michael Swann Building, Max Born Crescent, Edinburgh, EH9 3BF UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Turner","sequence":"additional","affiliation":[{"name":"Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, Michael Swann Building, Max Born Crescent, 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links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here, we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was increased in the absence of the Bre5-Ubp3 ubiquitin protease complex. Bre5 binds RNA in vivo, with a preference for exon 2 regions of intron-containing pre-mRNAs and poly(A) proximal sites. Ubiquitinated RNAPII showed similar enrichment. The absence of Bre5 led to impaired splicing and defects in RNAPII elongation in vivo on a splicing reporter construct. Strains expressing RNAPII with a K1246R mutation showed reduced co-transcriptional splicing. We propose that ubiquinitation of RNAPII is induced by RNA processing events and linked to transcriptional pausing, which is released by Bre5-Ubp3 associated with the nascent transcript.<\/jats:p>","DOI":"10.7554\/elife.27082","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T08:00:24Z","timestamp":1507881624000},"source":"Crossref","is-referenced-by-count":20,"title":["RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit"],"prefix":"10.7554","volume":"6","author":[{"given":"Laura","family":"Milligan","sequence":"first","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8226-7272","authenticated-orcid":true,"given":"Camille","family":"Sayou","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, University of 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tested a combination of microplanning (peer-led risk-differentiated support) and self-help groups (SHGs) to reduce the proportion of female sex workers (FSW) at risk of acquiring or transmitting HIV infection in Zimbabwe. The trial found overall high levels of FSW engagement with treatment which further improved in intervention sites. HIV prevention uptake, however, was low and not affected by AMETHIST.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We conducted a mixed methods process evaluation to assess implementation, explore FSW perceptions, and understand the trial\u2019s outcomes. We integrated routine programme statistics, qualitative data, and an endline respondent driven sampling survey among 4444 FSW to consider fidelity of implementation, feasibility of delivery, and acceptability. We also examined whether the intervention triggered changes hypothesised in its programme theory.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Microplanning was successfully introduced, with peers effectively providing risk-differentiated support. Early difficulties related to mapping sex work \u201chotspots,\u201d, maintaining contact with mobile FSW, and some resistance to regular risk assessments, but double the number of new FSW registered at intervention clinics compared to comparison sites (8443 v 3824), and significantly more HIV tests were performed (11882 vs. 6808). SARS-COV2 disrupted the intervention, particularly SHG. Fewer groups were established than planned, and lockdowns prevented group members meeting in person and participating in collective activities. Nonetheless, 30 of 65 established SHG remained active after two years, and more SHG members registered with clinics than those reached by microplanning alone (82 cv 76% p,0.001). Increased service use did not increase effective prevention. Over 80% FSW reported condomless sex and PrEP adherence was insufficient to achieve protection. Qualitative data show FSW prefer to \u201cwait\u201d until HIV seroconversion before taking daily medication. They feared conflation of ART with PrEP and resulting stigma. Concerns about side effects and immediate financial priorities undermined perceived future benefits of PrEP and condoms.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Strengthening HIV prevention should consider how to improve FSW\u2019s hope and agency alongside reducing stigma and supporting collective rights and action.<\/jats:p>\n <\/jats:sec>\n \n Trial registration<\/jats:title>\n The Pan African Clinical Trials Registry registered the trial 2nd July 2020 (PACTR202007818077777) which was after randomisation but before any research data were collected.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12913-026-14137-6","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T05:17:11Z","timestamp":1770441431000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u201cWe carried her in a wheelbarrow to the clinic\u201d: process evaluation of the AMETHIST intervention combining microplanning with self-help groups to improve HIV prevention and treatment among female sex workers in Zimbabwe"],"prefix":"10.1186","volume":"26","author":[{"given":"Fortunate","family":"Machingura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gracious","family":"Madimutsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Memory","family":"Makamba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatenda","family":"Kujeke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Takaruza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaspar","family":"Maguma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanni","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungai T.","family":"Chabata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Primrose","family":"Matambanadzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Steen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Shahmanesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amon","family":"Mpofu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen","family":"Mugurungi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew N.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Hargreaves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances M.","family":"Cowan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Busza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,7]]},"reference":[{"issue":"S3","key":"14137_CR1","doi-asserted-by":"publisher","first-page":"e25716","DOI":"10.1002\/jia2.25716","volume":"24","author":"H Jin","year":"2021","unstructured":"Jin H, Restar A, Beyrer C. 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Written informed consent from participants was obtained before enrolment.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"334"},{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:09:10Z","timestamp":1772168950280,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.73900.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000}},{"DOI":"10.12688\/f1000research.73900.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000}},{"DOI":"10.12688\/f1000research.73900.3","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000}}],"reference-count":32,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["214185\/Z\/18\/Z"],"award-info":[{"award-number":["214185\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["103994\/Z\/14\/Z"],"award-info":[{"award-number":["103994\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["074833\/Z\/04\/Z"],"award-info":[{"award-number":["074833\/Z\/04\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wellcome","award":["20517\/Z\/16\/Z"],"award-info":[{"award-number":["20517\/Z\/16\/Z"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"Background The long-term impact of elevated blood pressure on mortality outcomes has been recently revisited due to proposed changes in cut-offs for hypertension. This study aimed at assessing the association between high blood pressure levels and 10-year mortality using the Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC-7) and the American College of Cardiology and the American Heart Association (ACC\/AHA) 2017 blood pressure guidelines. Methods Data analysis of the PERU MIGRANT Study, a prospective ongoing cohort, was used. The outcome of interest was 10-year all-cause mortality, and exposures were blood pressure categories according to the JNC-7 and ACC\/AHA 2017 guidelines. Log-rank test, Kaplan-Meier and Cox regression models were used to assess the associations of interest controlling for confounders. Hazard ratios (HR) and 95% confidence intervals (95% CI) were estimated. Results A total of 976 records, mean age of 60.4 (SD: 11.4), 513 (52.6%) women, were analyzed. Hypertension prevalence at baseline almost doubled from 16.0% (95% CI 13.7%\u201318.4%) to 31.3% (95% CI 28.4%\u201334.3%), using the JNC-7 and ACC\/AHA 2017 definitions, respectively. Sixty-three (6.4%) participants died during the 10-year follow-up, equating to a mortality rate of 3.6 (95% CI 2.4\u20134.7) per 1000 person-years. Using JNC-7, and compared to those with normal blood pressure, those with pre-hypertension and hypertension had 2.1-fold and 5.1-fold increased risk of death, respectively. Similar mortality effect sizes were estimated using ACC\/AHA 2017 for stage-1 and stage-2 hypertension. Conclusions Blood pressure levels under two different definitions increased the risk of 10-year all-cause mortality. Hypertension prevalence doubled using ACC\/AHA 2017 compared to JNC-7. The choice of blood pressure cut-offs to classify hypertension categories need to be balanced against the patients benefit and the capacities of the health system to adequately handle a large proportion of new patients.<\/ns5:p>","DOI":"10.12688\/f1000research.73900.4","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T07:10:12Z","timestamp":1700464212000},"page":"1134","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Blood pressure and 10-year all-cause mortality: Findings from the PERU MIGRANT Study"],"prefix":"10.12688","volume":"10","author":[{"given":"Aida","family":"Hidalgo-Benites","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Senosain-Leon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo M.","family":"Carrillo-Larco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Ruiz-Alejos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam","family":"Smeeth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4738-5468","authenticated-orcid":false,"given":"J. Jaime","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6834-1376","authenticated-orcid":false,"given":"Antonio","family":"Bernab\u00e9-Ortiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1603-1658","DOI":"10.1016\/S0140-6736(16)31460-X","article-title":"Global, regional, and national disability-adjusted life-years (DALYs) for 315 diseases and injuries and healthy life expectancy (HALE), 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015.","volume":"388","year":"2016","journal-title":"Lancet."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2224-2260","DOI":"10.1016\/S0140-6736(12)61766-8","article-title":"A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990\u20132010: a systematic analysis for the Global Burden of Disease Study 2010.","volume":"380","author":"S Lim","year":"2012","journal-title":"Lancet."},{"key":"ref4","first-page":"37-55","article-title":"Worldwide trends in blood pressure from 1975 to 2015: a pooled analysis of 1479 population-based measurement studies with 19.1 million participants.","volume":"389","year":"2017","journal-title":"Lancet."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1206-1252","DOI":"10.1161\/01.HYP.0000107251.49515.c2","article-title":"Seventh report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure.","volume":"42","author":"A Chobanian","year":"2003","journal-title":"Hypertension."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"e426-e483","DOI":"10.1161\/CIR.0000000000000597","article-title":"2017 ACC\/AHA\/AAPA\/ABC\/ACPM\/AGS\/APhA\/ASH\/ASPC\/NMA\/PCNA Guideline for the Prevention, Detection, Evaluation, and Management of High Blood Pressure in Adults: Executive Summary: A Report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines.","volume":"138","author":"P Whelton","year":"2018","journal-title":"Circulation."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"956-960","DOI":"10.1016\/j.amjmed.2017.12.049","article-title":"Baseline Blood Pressure, the 2017 ACC\/AHA High Blood Pressure Guidelines, and Long-Term Cardiovascular Risk in SPRINT.","volume":"131","author":"M Vaduganathan","year":"2018","journal-title":"Am. 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AB-O (103994\/Z\/14\/Z) and JJM (074833\/Z\/04\/Z, 20517\/Z\/16\/Z) were supported by the Wellcome Trust, London, UK. 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AVATAR therapy involves voice-hearers engaging in a series of facilitated dialogues with a digital embodiment of the distressing voice. This randomized phase 2\/3 trial assesses the efficacy of two forms of AVATAR therapy, AVATAR-Brief (AV-BRF) and AVATAR-Extended (AV-EXT), both combined with treatment as usual (TAU) compared to TAU alone, and conducted an intention-to-treat analysis. We recruited 345 participants with psychosis; data were available for 300 participants (86.9%) at 16 weeks and 298 (86.4%) at 28 weeks. The primary outcome was voice-related distress at both time points, while voice severity and voice frequency were key secondary outcomes. Voice-related distress improved, compared with TAU, in both forms at 16 weeks but not at 28 weeks. Distress at 16 weeks was as follows: AV-BRF, effect \u22121.05 points, 96.5% confidence interval (CI)\u2009=\u2009\u22122.110 to 0,\n P<\/jats:italic>\n \u2009=\u20090.035, Cohen\u2019s\n d<\/jats:italic>\n \u2009=\u20090.38 (CI\u2009=\u20090 to 0.767); AV-EXT \u22121.60 points, 96.5% CI\u2009=\u2009\u22123.133 to \u22120.058,\n P<\/jats:italic>\n \u2009=\u20090.029, Cohen\u2019s\n d<\/jats:italic>\n \u2009=\u20090.58 (CI\u2009=\u20090.021 to 1.139). Distress at 28 weeks was: AV-BRF, \u22120.62 points, 96.5% CI\u2009=\u2009\u22121.912 to 0.679,\n P<\/jats:italic>\n \u2009=\u20090.316, Cohen\u2019s\n d<\/jats:italic>\n \u2009=\u20090.22 (CI\u2009=\u2009\u22120.247 to 0.695); AV-EXT \u22121.06 points, 96.5% CI\u2009=\u2009\u22122.700 to 0.586,\n P<\/jats:italic>\n \u2009=\u20090.175, Cohen\u2019s\n d<\/jats:italic>\n \u2009=\u20090.38 (CI\u2009=\u2009\u22120.213 to 0.981). Voice severity improved in both forms, compared with TAU, at 16 weeks but not at 28 weeks whereas frequency was reduced in AV-EXT but not in AV-BRF at both time points. There were no related serious adverse events. These findings provide partial support for our primary hypotheses. AV-EXT met our threshold for a clinically significant change, suggesting that future work should be primarily guided by this protocol. 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Clinical trial evidence to date is inconclusive. Favipiravir has been recommended for the treatment of COVID-19 in some countries.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In a multicentre open-label, randomised, controlled, adaptive platform trial, low-risk adult patients with early symptomatic COVID-19 were randomised to one of ten treatment arms including high dose oral favipiravir (3.6g on day 0 followed by 1.6g daily to complete 7 days treatment) or no study drug. The primary outcome was the rate of viral clearance (derived under a linear mixed-effects model from the daily log\n 10<\/jats:sub>\n viral densities in standardised duplicate oropharyngeal swab eluates taken daily over 8 days [18 swabs per patient]), assessed in a modified intention-to-treat population (mITT). The safety population included all patients who received at least one dose of the allocated intervention. This ongoing adaptive platform trial was registered at ClinicalTrials.gov (NCT05041907) on 13\/09\/2021.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n In the final analysis, the mITT population contained data from 114 patients randomised to favipiravir and 126 patients randomised concurrently to no study drug. Under the linear mixed-effects model fitted to all oropharyngeal viral density estimates in the first 8 days from randomisation (4,318 swabs), there was no difference in the rate of viral clearance between patients given favipiravir and patients receiving no study drug; a -1% (95% credible interval: -14 to 14%) difference. High dose favipiravir was well-tolerated.<\/jats:p>\n <\/jats:sec>\n \n Interpretation<\/jats:title>\n Favipiravir does not accelerate viral clearance in early symptomatic COVID-19. 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Measuring resistance in commensal bacteria could provide an objective indicator for evaluating the impact of interventions to reduce antibiotic use and resistance. This study aimed to evaluate the feasibility, acceptability, and bacterial recovery for different sampling strategies. We conducted a cross-sectional mixed methods study in a rural community in Ha Nam Province, northern Vietnam, and collected structured interviews, samples, and in-depth interviews from households. Out of 389 households invited, 324 participated (83%), representing 1502 individuals. Samples were collected from these individuals (1498 stool, 1002 self-administered nasal swabs, and 496 HW-administered nasopharyngeal swabs). Pneumococci were recovered from 11.1% (128\/1149) of the total population and 26.2% (48\/183) of those under 5-years. Recovery was higher for health-worker (HW)-administered swabs (13.7%, 48\/350) than self-administered swabs (10.0%, 80\/799) (OR 2.06, 95% CI 1.07\u20133.96).\u00a0Cost\u00a0per swab was\u00a0cheaper for self-administered ($7.26) than HW-administered\u00a0($8.63) swabs, but the overall\u00a0cost for 100 positive samples was higher ($7260 and\u00a0$6300 respectively).\ufeff Qualitative interviews revealed that HW-administered nasopharyngeal swabs took longer to collect, caused more discomfort, and were more difficult to take from children. Factors affecting participation included sense of contribution, perceived trade-offs between benefits and effort, and peer influence. Reluctance was related to stool sampling and negative perceptions of research. This study provides important evidence for planning community-based carriage studies, including cost, logistics, and acceptability. Self-administered swabs had lower recovery, and though cheaper and quicker, this would translate to higher costs for large population-based studies. Recovery might be improved by swab-type, transport medium, and better cold-chain to lab.<\/jats:p>","DOI":"10.1038\/s41598-022-27084-z","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T06:02:48Z","timestamp":1672293768000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Feasibility, acceptability, and bacterial recovery for community-based sample collection to estimate antibiotic resistance in commensal gut and upper respiratory tract bacteria"],"prefix":"10.1038","volume":"12","author":[{"given":"Hoang Huy","family":"Tran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hien Anh Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huyen Bang","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bich Ngoc Thi","family":"Vu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu Cam Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Costanza","family":"Tacoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao Phuong","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tung Son","family":"Trinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thien Huong Ngoc","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Behzad","family":"Nadjm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieu H\u01b0\u01a1ng Thi","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quynh Dieu","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Th\u01b0\u01a1ng Hong Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trang Thu","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thai Duy","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Kesteman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duc Anh","family":"Dang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tien Dac","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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This study evaluated the sequential effects of prior infection, heterologous boosting with mRNA-1273 (Moderna), and the occurrence of Omicron vaccine-breakthrough infection (VBI) thereafter.<\/jats:p>\n <\/jats:sec>\n Methods<\/jats:title>\n We evaluated anti-spike IgG (Abbott) and neutralising (cPASS\/GenScript) antibody (nAb) titers up to one year after mRNA-1273 boost in two-dose-CoronaVac-primed Indonesian healthcare workers (August 2021-August 2022). We used linear mixed modeling to estimate the rate of change in antibody levels, and logistic regression to examine associations between antibody levels and VBI.<\/jats:p>\n <\/jats:sec>\n Results<\/jats:title>\n Of 138 participants, 52 (37.7%) had a prior infection and 78 (56.5%) received an mRNA-1273 booster. After two-dose CoronaVac, antibody titers had significantly declined within 180\u00a0days, irrespective of prior infection. After mRNA-1273 booster, anti-spike IgG (1.47% decline\/day) and Omicron B.1.1.529\/BA.2 nAbs declined between day 28\u201390, and IgG titers plateaued between day 90\u2013360. During the BA.1\/BA.2 wave (February\u2013March 2022), 34.6% (27\/78) of individuals experienced a VBI (median 181\u00a0days after mRNA-1273), although none developed severe illness. VBI was associated with low pre-VBI anti-spike IgG and B.1.1.529\/BA.2 nAbs, which were restored post-VBI.<\/jats:p>\n <\/jats:sec>\n Conclusions<\/jats:title>\n mRNA-1273 booster after two-dose CoronaVac did not prevent BA.1\/BA.2 VBI. Periodic vaccine boosters may be warranted against emerging SARS-CoV-2 variants.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12879-024-09644-y","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T09:03:13Z","timestamp":1722502993000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Anti-SARS-CoV-2 antibody dynamics after primary vaccination with two-dose inactivated whole-virus vaccine, heterologous mRNA-1273 vaccine booster, and Omicron breakthrough infection in Indonesian health care workers"],"prefix":"10.1186","volume":"24","author":[{"given":"Suwarti","family":"Suwarti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilbert","family":"Lazarus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabighoh","family":"Zanjabila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Sinto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fransiska","family":"Fransiska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresia","family":"Deborah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dwi","family":"Oktavia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junaidah","family":"Junaidah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santayana","family":"Santayana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Surendra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeng","family":"Yuliana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herlina","family":"Pardosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nunung","family":"Nuraeni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saraswati","family":"Soebianto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Novi Dwi","family":"Susilowati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Decy","family":"Subekti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariel","family":"Pradipta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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To achieve the World Health Organization\u2019s goals for HCV elimination, there is a need for substantial scale-up in testing, treatment, and a reduction in new infections. Data on the population impact of scaling up treatment is not available in Pakistan, nor is there reliable data on the incidence of infection\/reinfection. This project will fill this gap by providing important empirical data on the incidence of infection (primary and reinfection) in Pakistan. Then, by using this data in epidemic models, the study will determine whether response rates achieved with affordable therapies (sofosbuvir plus daclatasvir) will be sufficient to eliminate HCV in Pakistan.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This prospective multi-centre cohort study will screen 25,000 individuals for HCV antibody (Ab) and RNA (if Ab-positive) at various centers in Pakistan- Karachi (Sindh) and Punjab, providing estimates of the disease prevalence. HCV positive patients will be treated with sofosbuvir and daclatasvir for 12-weeks, (extended to 24-weeks in those with cirrhosis) and the proportion responding to this first-line treatment estimated. Patients who test HCV Ab negative will be recalled 12\u00a0months later to test for new HCV infections, providing estimates of the incidence rate. Patients diagnosed with HCV (~\u20094,000) will be treated and tested for Sustained Virological Response (SVR). Questionnaires to assess risk factors, productivity, health care usage and quality of life will be completed at both the initial screening and at 12-month follow-up, allowing mathematical modelling and economic analysis to assess the current treatment strategies. Viral resistance will be analysed and patients who have successfully completed treatment will be retested 12\u00a0months later to estimate the rate of re-infection.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The HepFREEPak study will provide evidence on the efficacy of available and widely used treatment options in Pakistan. It will also provide data on the incidence rate of primary infections and re-infections. Data on incidence risk factors will allow us to model and incorporate heterogeneity of risk and how that affects screening and treatment strategies. These data will identify any gaps in current test-and-treat programs to achieve HCV elimination in Pakistan.<\/jats:p>\n <\/jats:sec>\n \n Study registration<\/jats:title>\n This study was registered on clinicaltrials.gov (NCT04943588) on June 29, 2021.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12889-023-17290-3","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T05:02:47Z","timestamp":1702875767000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HepFREEPak: protocol for a multi-centre, prospective observational study examining efficacy and impact of current therapies for the treatment of hepatitis C in Pakistan and reporting resistance to antiviral drugs: study protocol"],"prefix":"10.1186","volume":"23","author":[{"given":"Ambreen","family":"Arif","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliya","family":"Hasnain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Auj","family":"Chaudhry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Asim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Nabeel","family":"Shafqat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer","family":"Altaf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor","family":"Saba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polychronis","family":"Kemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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This typically involves manual delineation to measure the rectus femoris cross-sectional area (RFCSA), which is a subjective, time-consuming, and laborious task that requires significant expertise. We aimed to develop and evaluate an AI tool that performs automated recognition and measurement of RFCSA to support non-expert operators in measurement of the RFCSA using muscle ultrasound. Twenty patients were recruited between Feb 2023 and July 2023 and were randomized sequentially to operators using AI (n\u2009=\u200910) or non-AI (n\u2009=\u200910). Muscle loss during ICU stay was similar for both methods: 26\u2009\u00b1\u200915% for AI and 23\u2009\u00b1\u200911% for the non-AI, respectively (\n p<\/jats:italic>\n \u2009=\u20090.13). In total 59 ultrasound examinations were carried out (30 without AI and 29 with AI). When assisted by our AI tool, the operators showed less variability between measurements with higher intraclass correlation coefficients (ICCs 0.999 95% CI 0.998\u20130.999 vs. 0.982 95% CI 0.962\u20130.993) and lower Bland Altman limits of agreement (\u00b1\u20091.9% vs. \u00b1\u20096.6%) compared to not using the AI tool. The time spent on scans reduced significantly from a median of 19.6 min (IQR 16.9\u201321.7) to 9.4 min (IQR 7.2\u201311.7) compared to when using the AI tool (\n p<\/jats:italic>\n \u2009<\u20090.001). AI-assisted muscle ultrasound removes the need for manual tracing, increases reproducibility and saves time. This system may aid monitoring muscle size in ICU patients assisting rehabilitation programmes.\n <\/jats:p>","DOI":"10.1038\/s41598-024-64564-w","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T15:21:54Z","timestamp":1719415314000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Clinical evaluation of AI-assisted muscle ultrasound for monitoring muscle wasting in ICU patients"],"prefix":"10.1038","volume":"14","author":[{"given":"Phung Tran Huy","family":"Nhat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen","family":"Van Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lam Minh","family":"Yen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen 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